Atp23p and Atp10p coordinate to regulate the assembly of yeast mitochondrial ATP synthase

Two chaperones, Atp23p and Atp10p, were previously shown to regulate the assembly of yeast mitochondrial ATP synthase, extra expression ATP23 was found partially rescue an atp10 deletion mutant, by unknown mechanism. Here, we identified that residues 112-115 (LRDK) required for its function in assisting demonstrated both functions Atp23p, processing subunit 6 precursor partial mutant. By chasing labeling with isotope 35S-methionine, stability synthase increased null strain upon overexpression ATP23. Further co-immunoprecipitation (Co-IP) blue native PAGE experiments showed Atp10p physically associated each other wild type. Moreover, revealed level mutant compared Furthermore, that, after 72 hours growth, leaky growth on respiratory substrates, presence low recovery oligomycin sensitivity ATPase activity. characterization 24 These results indicated, ATP10 deficiency could be complemented stabilizing some synthase. Taken together, this study two chaperones coordinated which advanced our understanding mechanism